Nick here from Shopify. Great question!
If I'm correct in understanding what you're looking for, I think it is possible. If it is the same store you wouldn't be able to use multiple payment gateways such as Stripe and then another gateway, but you are able to accept payment in different ways, such as by credit card, cash etc.
Shopify has a few helpful guides which can show you the different ways you can accept payment in physical sales retail locations:
Hopefully, this helps and answers what you're looking for?
Nick | Community Moderator @Shopify
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